Bzr1 chip-seq
WebFeb 18, 2010 · The ChIP procedure is optimized for high signal-to-noise ratio starting with tissue fixation, followed by nuclei isolation, immunoprecipitation, DNA amplification and purification, and the complete protocol for ChIP-SEQ/ChIP-CHIP sample preparation takes ∼7 d. Chromatin immunoprecipitation (ChIP) is a powerful technique to study interactions …
Bzr1 chip-seq
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WebTo generate comparable data sets, we performed BZR1 ChIP-Seq analysis using dark-grown seedlings (Figure 1—source data 1B), as used in the ARF6 and PIF4 ChIP-Seq (Oh et al., 2012). Interestingly, large portions of ARF6 binding targets overlapped with either tar-gets of BZR1 (51%) or PIF4 (71%) or both (42%) (Figure 1C). The common targets ... WebChIP and RNA-seq analysis showed ZmBZR1 can directly bind to the promoter region of organ size related genes, Germination Repression and Cell Expansion receptor-like …
WebJan 18, 2024 · The genome-wide ChIP-Seq analysis revealed that ARF6 shares a vast number of genomic targets (around 50%) with BZR1 and the light/temperature-regulated transcription factor PIF4 by CHIP-Seq analyses (Oh et al., 2014). BZR1 and PIF4 interact with ARF6 and activate shared target genes by binding to shared target genes … WebNov 27, 2024 · Yeast cells with positive DNA-protein interactions were grown on Leu − plates with 100 ng mL −1 aureobasidin A. C, Direct binding of BZR1 to the promoters of ATG2 and ATG6 was investigated using ChIP-qPCR in BZR1OE plants. Six-week-old BZR1OE plants were treated with water or 500 n m BL, and input chromatin was isolated …
WebAug 13, 2024 · We generated a first-generation (T 0) transgenic CRISPR-bzr1 line. PCR genotyping and sequencing identified a deletion of 42 bp in proximity to single guide RNA (sgRNA) target sequences ... (E, F) ChIP-qPCR assay of BZR1 binding to the promoters of FER2 and FER3 genes in control and heat stress conditions. WebAug 4, 2024 · Comparing PIF-, BZR1-, HY5-, and MYC-Related ChIP-seq and RNA-seq Data Although DNA ChIP-seq data indicate a central role for PIF4 by binding to the …
WebApr 20, 2015 · Comparison of genes upregulated or downregulated by BR or auxin, identified by RNA-seq in roots (Table S1), to the BZR1 ChIP-chip and ChIP-seq data, and the RootMap spatial gene expression data. QC, quiescent center; MZ, meristem zone; TEZ, transition-elongation zone; LEZ, late elongation zone; DZ, differentiation zone.
Web56371 Ensembl ENSG00000105325 ENSMUSG00000020245 UniProt Q9UM11 Q9R1K5 RefSeq (mRNA) NM_001136197 NM_001136198 NM_016263 NM_019757 RefSeq … corporate lawyer salary san franciscoWebMar 11, 2005 · Lane S is the G + A chemical sequencing reaction. Footprint regions are marked by vertical lines and corresponding sequence. (D) RBSS for optimal BZR1 binding site. Shown on the top is the alignment of all identified sequences, with the matching sequences marked in red. ... we performed chromatin immunoprecipitation (ChIP) … farberware stainless steel pans inductionWebJefferson County, MO Official Website corporate lawyer salary san diegoWebThe function of BZR1 on organ size is well characterized in Arabidopsis, but poorly understood in maize (Zea mays). ... ChIP and RNA-seq analysis showed ZmBZR1 can directly bind to the promoter region of organ size related genes, Germination Repression and Cell Expansion receptor-like kinase (GRACE) and KIP-RELATED PROTEIN6 … corporate lawyer salary rangeWebMay 20, 2024 · Chromatin immunoprecipitation followed by sequencing (ChIP-seq) in B73xMo17 F1s identified thousands of target genes of ZmBZR1. Allele-specific ZmBZR1 binding (ASB) was observed for about 14.3% of ... farberware stainless steel tea kettleWebLists of BR- and auxin-responsive genes and BZR1 target genes. Comparison of genes up- or down-regulated by BR or auxin, identified by RNA-Seq in roots (Table 2-S1), to the BZR1 ChIP-chip and ChIP-Seq data [S1, S2], and the RootMap spatial gene expression data [S3]. QC, quiescent center; MZ, meristem zone; TEZ, transition-elongation zone; LEZ ... farberware stainless steel roasting panWebMay 27, 2014 · ChIP-Seq analysis R (CSAR) software was used to identify binding peaks, with parameters (backg = 10, norm = −1, test = 'Ratio', … corporate lawyer salt lake city