Cells treated with jasplakinolide
Webcells treated with LatB and Jasplakinolide (Figures S1K,L). Flagellar regeneration was also impaired in cells treated with latB or Jasplakinolide (Figure S1M). When flagella regenerate, new flagellar proteins are synthesized and then transported into the flagellum by IFT. Impairment of either precursor pool regeneration or IFT could cause WebA, control cells; B–D, cells treated for 2 h with 50 n m jasplakinolide (B); 100 n m jasplakinolide (C), and 200 n m jasplakinolide (D). E–H, …
Cells treated with jasplakinolide
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WebAug 31, 2011 · In experiments containing blebbistatin, cells were preincubated in 100 nM fMLP and 50 μM blebbistatin for 10 min. Cells were then treated with either solutions of latrunculin B or jasplakinolide to achieve final concentrations of 100 nM fMLP, 50 μM blebbistatin, and 500 nM latrunculin B, or 100 nM fMLP, 50 μM blebbistatin, and 1 μM ... WebOct 13, 2024 · To investigate the role of filamentous actin in the endocytic pathway, we used the cell-permeant drug Jasplakinolide (JAS) to polymerize actin in intact polarized Madin–Darby canine kidney (MDCK) cells. The uptake and accumulation of the fluid-phase markers fluorescein isothiocyanate (FITC)-dextran and horseradish peroxidase (HRP) …
WebApr 13, 2024 · Of these, host cell invasion is the best-characterised actin-dependent process (reviewed in ). In P. falciparum, naturally occurring compounds such as cytochalasins D and B, latrunculins, phalloidin, and jasplakinolide have been used to study the complex actin regulation in merozoite invasion [7,29,45–48]. These compounds … WebNov 30, 2012 · Jasplakinolide or DMSO-treatment did not activate caspase-3 in late EPCs cultured with VEGF. However caspase-3-like activity was present in both jasplakinolide and DMSO-treated EPCs after 6 h …
WebJasplakinolide was added to the cells at final con-centrations of 50–300 nM, and cells were examined over a 1–24-h period. For F-actin staining treated and untreated cells were washed 23 in PBS, fixed with 3.7% formaldehyde in PBS for 15 min at 21 °C, washed 33 with PBS, and permeabilized by dipping in acetone at 220 °C for 5 min. WebMay 1, 2008 · In this study, we report the formation of several cytoplasmic inclusion bodies composed of filamentous actin (F-actin) and generated by experimental treatments using depolymerizing or stabilizing actin toxins in neuronal and non-neuronal mammalian cell lines. The actin-stabilizing toxin jasplakinolide (Jpk) induced, in a microtubule …
WebJasplakinolide (Jaspamide);≥97% HPLC; Jasplakinolide has been used to analyze its influence on sciatic nerve guidance effect in vivo in chicken embryos; Jasplakinolide is …
WebThe initial response of Listeria motility in jasplakinolide-treated PtK2 cells was very similar to protrusion in migrating fibroblasts. For both systems, there was a rapid decrease in the rate of motility, on average to less than one half of the control by 3 minutes of treatment with jasplakinolide (Figure 6, compare circles and diamonds; Table 1). mock tops for womenWebMay 22, 2015 · Treatment of control and TREK-1 deficient cells with TNF-α had no effect on F-actin filaments when compared to untreated cells (Fig 1D and 1I), and the combination of TNF-α+cytochalasin D or TNF-α+jasplakinolide had no additional effect on F-actin filaments when compared with cytochalasin D or jasplakinolide alone, respectively. To … mock top shells automobileWebJan 1, 2009 · As competitive binding inhibition of phalloidin to F-actin by jasplakinolide is described , and decreased FITC-phalloidin labeling is noted as a consequence of jasplakinolide treatment in MDCK cells , the … inlite led ceciaaWebJan 14, 2024 · For jasplakinolide-treated cells, the actin filaments were not as distinct as in control cells. However, an increase in the signal of β-actin, especially at the cell … mock toursWebMar 10, 2015 · Treatment of MCF-7 cells with jasplakinolide decreased E-cadherin expression suggesting that actin polymerizing and depolymerizing events have opposing effects on EMT. Interestingly, Cyt D effect seemed specific to cancer cells suggesting that small molecule regulators of actin cytoskeleton might be potentially developed as … mock tower busch gardensWebFeb 12, 1999 · To confirm that jasplakinolide altered the actin cytoskeleton in CTLL-20 cells, cells were treated with jasplakinolide (100 n m) or Me 2 SO (0.02%) for 8 h in … mock tops for ladiesWebFeb 18, 2000 · Jasplakinolide is a cyclic peptide isolated from the marine sponge, Jaspis johnstoni, that we have previously shown to bind to and stabilize filamentous actin in vitro (1). In vivo data suggests that jasplakinolide-treated prostate cancer cells have both decreased labeling of F-actin and decreased amounts of rhodamine-phalloidin bound to … in literature a symbol is something