WebPepMute™ siRNA Transfection Reagent, formulated from simulation of virus cell penetrating peptides (CPPs), is a total novel siRNA delivery tool which provides more than 95% silencing efficiency at 1 nM siRNA in variety of mammalian cells. WebSep 28, 2012 · This is vastly superior to a commercially available control, DharmaFECT, which resulted in only ∼60% siRNA positive MSCs. Moreover, the diblock copolymer, at conditions that result in excellent knockdown (down to ∼10% of control gene expression), was cytocompatible, causing no negative effects on MSC survivability.
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WebThe siRNA was added to the DharmaFECT transfection reagent and incubated for 20 minutes at room temperature. Antibiotic-free complete medium (1,600 μL) was then added. Finally, the culture medium was removed from the wells of the six-well plates and 2 mL of the appropriate transfection medium was added to each well. WebFunctional Co-transfection of Plasmid DNA and siRNA Using the TransIT-X2® Dynamic Delivery System. TransIT-X2® Dynamic Delivery System was used to transfect plasmid Cy®5 labeled DNA encoding nuclear YFP and Cy®3 labeled siRNA into HeLa cells.Transfection was performed in 6-well plates with Poly-L-Lysine (PLL) coated … raymond bunn
Dharmacon™ DharmaFECT™ 1–4 transfection …
WebCell line Cell type Recommended DharmaFECT formulation DharmaFECT volume/well (µL)Plating density Other successful formulations Rodent A7R5 Rat aortic smooth muscle 2 0.1 5 x 103 1 C2C12 Mouse ... WebDharmaFECT® Duo is a lipid-based reagent specially formulated for co-transfection of plasmid and siRNA. Under optimized conditions, efficient delivery of both plasmid and siRNA can be achieved with minimal cell toxicity. As is generally observed with plasmid transfection, cell viability is reduced compared to siRNA transfection alone. Webrange of DharmaFECT™ transfection reagent volumes. In this example, U2OS Ubi[G76V]-EGFP-Cas9 cells were trypsinized, then diluted to 5000, 10000 or 20000 cells per 80 μL in growth medium. Edit-R PPIB synthetic crRNA Control (Cat #UK-007050-01) was used as a positive control for gene editing and simplicity halloween costumes catalog