WebApr 27, 2024 · Recently, an RNA transcript-template homology-directed DNA repair (TT-HDR) strategy was developed to overcome the obstacle of delivery of DRT into plant cells to repair DSBs through HDR, taking advantage of the fact that RNA transcripts can be produced abundantly in vivo ( Li et al., 2024 ). WebApr 7, 2024 · Rhodococcus biphenylivorans GA1 was successfully isolated, which can efficiently degrade alkali lignin and a variety of lignin-derived aromatic compounds as the sole carbon source. Whole genome sequencing of strain GA1 showed that it possessed G + C content of 68% with the size of 6.0 Mb and 4319 putative open reading frames (ORFs). …
Engineering crops of the future: CRISPR approaches to
WebFeb 18, 2024 · As a key element of genome editing, donor DNA introduces the desired exogenous sequence while working with other crucial machinery such as CRISPR-Cas or recombinases. However, current methods for the delivery of donor DNA into cells are both inefficient and complicated. Here, we developed a new methodology that utilizes rolling … WebDec 6, 2024 · A few resistance genes, located on mobile genetic elements, were found. Several plasmids were detected, though only one was carrying resistance genes. This is the first study on the prevalence of Salmonella in pigs in the Caribbean and that used whole genome sequencing for characterization. The strains were rather susceptible. butchers hill association
Cells Free Full-Text Dynamic Genome Editing Using In Vivo ...
WebCRISPR/Cas is in the process of inducing the biggest transformation of plant breeding since the green revolution. ... of inducing directed changes by homologous recombination have been improved significantly and strategies to break genetic linkages by inducing chromosomal rearrangements have been developed. Cas13 systems have been applied … WebThe ability to precisely modify DNA in cells offers great opportunities for basic and applied research, yet it remains difficult to achieve for most plant species. Baltes et al. (pages … WebApr 10, 2024 · Replicons contain a partial genome, including cis-acting elements in the 5′- and 3′-ends as well as the genes of all non-structural proteins under the control of cytomegalovirus (CMV) immediate-early promoter. The CMV promoter driven viral RNA subgenomes enable self-replication in transfected cells but have no infectious particles [ … cctv camera mounting boxes