Web2 days ago · Western blot analysis was conducted to measure the protein levels of CDK1 and Cyclin B in BeWo cells. GAPDH serves as loading control. Data are expressed as the mean ± standard deviation (SD). *P < 0.05, ***P < 0.001. E. Western blot analysis was conducted to measure the protein levels of CDK2 and p-CDK2 in BeWo cells. WebIn this study, we investigated the potential role of neuregulin receptor degradation protein-1 (Nrdp1), a regulator of ErbB3 protein stability, in HCC progression. Methods: We compared the expression of Nrdp1 in various HCC cell lines and in 8 pairs of tumor and peritumor tissue samples using Western blot analysis.
GAPDH antibody (10494-1-AP) Proteintech - ptglab
Web1:5000 for the secondary may be too high. Try reducing the secondary to 1:20000 or higher, and check whether the 2 bands show up after longer exposures. Try with higher gel … WebWestern blot analysis of extracts from OVCAR8, HT29 and Jurkat cells using Bak Antibody. Simple Western™ analysis of lysates (1.0 mg/mL) from Jurkat cells using Bak Antibody #3814. The virtual lane view (left) shows a single target band (as indicated) at 1:10 and 1:50 dilutions of primary antibody. sports direct phone holder run
Low weight protein degradation during western blot - Research…
WebAntibodies for western blotting are typically used as dilute solutions, and manufacturers may recommend using ranges from a 1/100–1/500,000 dilution from a 1 mg/mL stock solution. However, the optimal dilution of a given antibody with a particular detection system must be determined experimentally. WebFull length native protein (purified) corresponding to Human GAPDH. Positive control This antibody gave a positive signal in the following whole cell lysates: HeLa; NIH3T3. This antibody also gave a positive signal in Human brain tissue lysate. General notes WebJan 29, 2015 · An important method in cardiovascular research is the extraction and detection of cytosolic and membrane proteins and proteins located in a sub-cellular organelle. The methods used for this... shelter discrimination