Web5 jul. 2024 · HY5 and the closely related HY5 HOMOLOG (HYH) accumulate rapidly following UV-B exposure as a result of protein stabilization and increased transcription, 10,26,27 and mediate transcription of many UVR8-target genes. 8,10,28 UVR8 monomers can re-associate to form dimers, 29,30 a process that is facilitated by the negative … WebThere were 49 upregulated metabolites and 41 downregulated metabolites under UV-B radiation. Flavonoids were the most significantly changed metabolites. qPCR analysis showed that UVR8-COP1-HY5 signaling pathway genes and photolyase genes (i.e., LpUVR3, LpPHR1, and LpDPL) were significantly up-regulated under UV-B light.

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Web31 aug. 2009 · HY5 is a key effector of responses mediated by UVR8. In mature leaf tissue, HY5 transcript accumulation occurred rapidly in response to a brief UV-B treatment and … Webfor UVR8 ChIP, we did not detect any specific HY5 sig-nal above background in YFP-UVR8 and CFP-UVR8 ChIP assays, a result that was mirrored in the non-transgenic negative controls (hy5-1, uvr8-7, Col WT) (Fig. 1e and Additional file 1c). To verify that YFP-UVR8, CFP-UVR8 and HY5-YFP were efficiently immunoprecipitated with the anti-GFP superstar tv programa

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WebUnder UV and blue lights, COP1/SPA is disrupted to suppress unnecessary and costly photomorphogenesis responses. UVR8 induces HY5 activity, and the binding of UVR8 to COP1 in UV-B disrupts PIF5 stabilization, rapidly lowering PIF5 abundance in sunlight by degradation via ubiquitination and proteasome-mediated protein degradation. Web1 feb. 2024 · In response to UV-B treatment, UVR8 interacts with COP1 with high affinity, which leads to dissociation of COP1-HY5 protein complex and HY5 protein stabilization [17, 18, 20, 22, 30]. UV-B induces UVR8 homodimer dissociation into monomer and post UV-B UVR8 monomer can reassemble into homodimer, forming a complete photocycle [ 31 , 32 ]. WebHY5 is regulated by UVR8 and required for UV-B protection. (A) HY5 and control ACTIN2 transcripts measured by RT-PCR in wild-type and uvr8-2 plants grown for 3 weeks in 25 … superstar tv program danas