NettetFigure 2. Mouse ear DNA rapid extraction and 2.5kb amplification using supplied PCR reagent PCRBIO Rapid Extract PCR Kit was used to extract DNA from 3mg of mouse ear clipping following the standard 15 minute protocol. The extraction was repeated using equivalent extraction kits from alternative manufacturers. A serial two fold dilution Nettet30. okt. 2015 · endobj xref 13 28 0000000016 00000 n 0000001216 00000 n 0000001371 00000 n 0000001819 00000 n 0000002224 00000 n 0000002684 00000 n 0000004241 00000 n 0000005470 00000 n 0000006643 00000 n 0000007929 00000 n 0000009186 00000 n 0000010460 00000 n 0000011591 00000 n 0000012731 00000 n 0000012844 …
PCRBIO Rapid Extract PCR Kit
Methods for permanently identifying mice include ear punching, ear tagging, tattooing, and subcutaneously implanting a numerically coded microchip. Ear punching, commonly used for identification of rodents, involves using a special punch to either produce a small (0.5-2 mm) notch near the edge of the ear … Se mer Several systems are available for individually identifying research mice. Depending on the circumstances, either temporary or permanent methods may be used. Se mer Several methods are particularly well suited for identifying cage mates. A felt tip marker may be used for marking an ear or tail; such marks usually … Se mer Resources for instruments Following are the names and addresses of some companies that sell animal identification tools. Other companies may also sell them. Ear punches 1. Roboz Surgical Instruments Company, … Se mer Nettetmouse femur. Neuroscience 113:155-166. 21. Mathias N, Robinson MA, Crook R, Lockworth CR, Goodwin BS. 2013. Local Cryoanalgesia Is Effective for Tail-Tip Biopsy … directions to ruth chris steakhouse
IACUC Policy 17: Tail Snipping/Clipping, Ear Punching and Toe …
NettetEar Punch . Placement of the ear punch hole can be used to identify animals as well as obtain a genotyping sample. 1. Restrain the animal by grasping the skin along its back … Nettetii. Ears must be big enough for the punch • This is generally 14 days old. iii. Numbering system may be difficult to read and discern. iv. Ear notch or punch tissue remnants may provide enough DNA for genotyping. Punch Procedure i. Gently restrain mouse with gloved hands by scruffing the dorsum high on the neck to control both the body and ... NettetOn the back side of the ear the number of perspiratory glands amounts to only 25% of the number in other parts of the body surface. In more than 50% of the retroauricular... directions to rutgers new brunswick