Psc101ts
WebLow copy number plasmid from Salmonella. pSC101 was the first plasmid vector used for cloning. WebSep 1, 2009 · Recombineering with Saccharomyces cerevisiae is a powerful methodology that can be used to clone multiple unmarked pieces of DNA to generate complex constructs with high efficiency. Here, we introduce two new tools that utilize the native recombination enzymes of S. cerevisiae to facilitate the manipulation of DNA. First, yeast …
Psc101ts
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WebThe latest tweets from @pSC101TS WebJan 7, 2024 · This plasmid is used for CRISPR mediated genome editing of E. coli growth strain : Constitutive expression of Cas9, inducible expression of the Red recombineering system, and inducible expression of the plasmid curing system for CRISPR mediated genome editing of E. coli. origin : 30 pi : plasmid copy :
Web3101C. Description. SWITCH TOGGLE SPDT 6A 125V. Manufacturer Standard Lead Time. 10 Weeks. Detailed Description. Toggle Switch SPDT Panel Mount. Customer Reference. … WebMay 22, 2015 · Since CRISPR/Cas9-coupled recombineering does not rely on the use of chromosome-encoded antibiotic resistance, or flippase recombination for antibiotic marker recycling, our approach is simpler, less labor-intensive, and allows efficient production of gene replacement mutants that are both markerless and "scar"-less. Free full text
WebpSC101ts from pGRG36 (Table S2). The gRNA 1 was amplified entirely from a previous construct (pKN02), while the spacers of gRNA 2 and 3 were added in PCR primer tails. For pKill3, assembly tags were placed be-tween each gRNAs to prevent miss-assembly due to the repetitive nature of this gene locus. Junctions between DNA segments were cre- WebThree E. coli strains, AB1157 (rec ), JC5519 (recB21 recC22), and BIK3686 (recC1002) carrying pIK172 (pSC101Ts, EcoRI r m , Ap r ) or pIK173 (its r version) were grown with aeration at 30°C in L...
WebDec 15, 2008 · I-CreI is encoded on a pSC101 plasmid with tetracycline resistance and a temperature sensitive origin of replication, and this construct is called pCURE. We initially tested the system by comparing growth on selective media of cells producing a well expressed membrane protein (GlpF) and those expressing a poorly expressed membrane …
WebJul 1, 2024 · Both plasmids were assembled using a similar strategy combining the cas9 gene from pCas9, the kanamycin resistance gene aph-IIIa from pKD4, and the oriVpSC101ts from pGRG36 (Table S2). The gRNA 1 was amplified entirely from a previous construct (pKN02), while the spacers of gRNA 2 and 3 were added in PCR primer tails. size 40 men shoes in usWebVarious E. coli strains carrying pIK172 (pSC101Ts, EcoRI r m , Ap r ) or pIK173 (its r version) were grown with selective antibiotics to log phase at 30°C and were then streaked on L agar for ... size 40 football jerseyWebFeb 13, 2016 · The pBBknock vector includes a pSC101ts temperature-sensitive origin (BBa_J107112) derived from pAH123 [ 21] [GenBank: AY048726] (see Additional file 1: Details about pBBknock thermosensitive sequence design). sus muffin songWebJournal: Applied Microbiology and Biotechnology Article Title: Comparison of engineered Escherichia coli AF1000 and BL21 strains for (R)- 3-hydroxybutyrate production in fed-batch cultivation doi: 10.1007/s00253-019-09876-y Figure Lengend Snippet: List of E. coli strains and plasmids used in this study Article Snippet: pSIJ8 , pKD46 derivative, temperature … size 40 is what us sizeWebJul 7, 2008 · PSC101 RepA protein can inhibit chromosomal DNA replication and induce the SOS response While studying the effects of RepA on pSC101 replication and partitioning … susnigdha tripathyWebApr 1, 2000 · Isogenic rec + and recBC strains with a sfiA::lacZ promoter fusion (BIK3920 and BIK3921) carrying pIK172 (pSC101Ts, EcoRI r + m +, Ap r) or pIK173 (its r − version) were aerated at 30°C in L broth with antibiotics and grown to an optical density at 660 nm (OD 660) of 0.3. Then the antibiotics were removed, and the culture was transferred to ... susnet sherbert cartridge reviewsWebJan 12, 2024 · Recombineering (short for homologous recombination-mediated genetic engineering) can be used to make an assortment of DNA modifications: insertion and … size 40 motocross pants